Laboratory of Recombinant Vaccines
Head: Prof. Bogusław Szewczyk
Beata Gromadzka, Ewelina Król PhD, Dawid Nidzworski PhD,
Łukasz Rąbalski PhD
Martyna Krejmer, Krzysztof Łepek, Paulina Weiher
The application of different expression systems for the production of viral
proteins which can be used as potential vaccines or constituents of modern
diagnostic tests is the main field of interest of the Laboratory. Apart from using
a wide array of classical methods of molecular biology, the Laboratory
specializes in expression of foreign genes in insect cells using baculovirus
expression system. Some of the research projects carried out in the Laboratory
are listed below:
1. Construction of recombinant vaccines against bird flu virus H5N1, classical
swine fever virus CSFV and rabbit hemorrhagic disease virus RHDV.
2. Effect of glycosylation inhibitors on viral entrance and propagation in host cells.
3. Fast and sensitive methods for the monitoring of influenza A and Newcastle
disease virus carried by migrating birds.
4. Fast and sensitive methods for the monitoring of baculoviruses controlling the populations of lepidopteran pests in forests.
Future projects and goals:
The influenza A/H1N1 responsible for the first pandemic of the twenty-first
century turned out to be relatively mild when compared to other influenza
pandemics of the past. However, such a situation may be transient and there
are concerns that the virus may reassort during mixed infections of a single host with different variants of influenza A virus. The resulting recombinant virus may have unpredictable properties when it establishes itself in the human population.
Therefore, our future goal is to develop fast molecular methods allowing rapid
identification of mixed infections in a single patient. Another of our aims is the
construction of a recombinant universal vaccine against influenza A/H1N1 virus.
Szewczyk B., Barski P., Sihler W., Rąbalski Ł., Skrzecz I., Hoyos-Carvajal L.,
Lobo de Souza M. Detection and identification of baculovirus pesticides by
multitemperature single-strand conformational polymorphism. J Env Sci Health
2008, 43: 539-545.
Gromadzka B., Śmietanka K., Dragun J., Minta Z., Góra-Sochacka A.,
Szewczyk B. Detection of changes in avian influenza genome fragments by
multitemperature single strand conformational polymorphism. Mol Cel Probes
2008, 22: 301-304.
Szewczyk, B., Rabalski, L., Krol, E., Sihler, W., Lobo de Souza, M. Baculovirus
biopesticides - a safe alternative to chemical protection of plants. J.
Biopesticides, 2009, 2: 209-216.
Adamiak B, Trybala E, Mardberg K, Johansson M, Liljeqvist JA, Olofsson S,
Grabowska A, Bienkowska-Szewczyk K, Szewczyk B, Bergstrom T. Human
antibodies to herpes simplex virus type 1 glycoprotein C are neutralizing and
target the heparan sulfate-binding domain. Virology, 2010, 400: 197-206
Krol E, Wandzik I, Szeja W, Grynkiewicz G, Szewczyk B. In vitro antiviral
activity of some uridine derivatives of 2-deoxy sugars against classical swine
fever virus. Antiviral Res., 2010, 86: 154-162.